PENGARUH VARIETAS DAN PENAMBAHAN ANTIVIRAL RIBAVIRIN PADA PENUMBUHAN JARINGAN MERISTEM BAWANG PUTIH (Allium sativum L)
Keywords:
Bawang putih (Allium sativum L), Antiviral Ribavirin, Meristem, Varietas
Abstract
Tanaman bawang putih (Allium sativum L) termasuk dalam genus Allium yang diperbanyak secara vegetatif melalui siung. Pada tanaman yang diperbanyak secara vegetatif, virus merupakan penyakit penting yang harus dipecahkan. Penanaman jaringan meristem dan penambahan antiviral Ribavirin merupakan salah satu cara untuk mengeliminasi virus. Penelitian dilaksanakan di Lab. Kultur jaringan Balai Penelitian Tanaman Sayuran pada bulan Maret s.d Mei 2019. Tujuan dari penelitian untuk melihat pengaruh dan perkembangan jaringan meristem 5 varietas Bawang putih (Varietas Lumbu Hijau, Lumbu Putih, Lumbu Kuning, Tawang Mangu, dan Sangga Sembalun) di media MS dengan penambahan Antiviral Ribavirin. Adapun perlakuan adalah 5 varietas bawang putih, konsentrasi Antiviral Ribavirin ( 0,5, 10 mg L-1). Hasil pengamatan secara visual penambahan antiviral Ribavirin berpengaruh pada perkembangan jaringan meristem 5 varietas bawang putih. Rata-rata jumlah daun, akar dan tinggi tanaman pada media MS dengan penambahan Antivira Ribavirin selalu lebih rendah dari tanpa perlakuan.
References
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Ammissah S, Collenmann P, Sintim H, Akromad R. 2016. In vitro control of microbial contamination of sweet potato cultured with nodal explants. In Annual Res. &Riview in Biology . vol 9 (3) 1-8.
Aqlima, Bambang S.P, Sri H.H dan Dinny D. 2018. Aplikasi ribavirin pada shoot tip bawang merah untuk eliminasi virus OYDV . J.Hort vol 28; 251 -258.
Armin , N.M; G.A. Wattimena; L. Winata 1992. Perbanyakan tanmana dalam bioteknologi tanaman dalam Wattimena et al (eds) PAU Bioteknologi IPB. Dirjen Dikti Dept. P&K. pp 12 – 18
Asih K.K and Neni Gunaeni. 2018. efek antiviran ribavirin dalam pertumbuhan dan perkembangan eksplant bawang putih cv. Lumbu hijau, cv Lumbu kuning dan cv. Tawangmangu. Agrin Vo. 22, P. 93 -103. ISSN 1410-0029.
Awan, A.R; Mughal S.M; Iftilhar Y and Kan. H.Z. 2007 . In vitro elimination of potato leaf roll virus from potato varieties. Euro. J. R. 18; 154 – 164.
Ayabe M. and Sumi S. 1988. Establishment of a novel tissue culture method , stem disc culture and its practical application to micropropagation of garlic (Allium sativum L). Plant cell culture Report. 17; 773 -779.
Bhojwani S.S and P.K. Datu. 2013. Production of virus free plants by tissue culture pp. 227 – 243. In S.S Bhojwani and P.K. datu (eds). Plant tissue culture. An Introduction to text. Springer India. DOI. 10 1007/979-81-322-1026-9
Bodani A and Chamka J.S. 2010. In vitro sterilization protocol for micropropagation of solanum tuberosum cv. Kufri Himalini . Academia Arena 2 ( 4) pp 24 – 27.
Dinarti D; Agus Purwito; Anas D,S dan Iis. R; 2008. Pembentukan umbi lapis mikro dua kultivar bawang merah (Allium cepa var Agregatum group). Pada beberapa konsentrasi sucsinic Acid Daminozide Hydrazide. J. Ilmu Pertanian Indonesia. April 2008; 32 – 37. ISSN 0853 – 4217.
Dugssa G and Feyissa T. 2011. In vitro production of virus free sweet potato (Ipomoe batatas L) by meristem culture and thermotheraphy. Etiop. J. Sci. 34 (1) 17 – 28.
Gamborg O.L. et al. 1976. Nutrient requirement of suspension culture of soybean root. Cell Exp. Res. 50 151 – 58.
Geier, T. 1990. Anthorium . In Ammirato , P.V; D.A. Evans; W.R. Sharp and Y.P. S. Bajaj (eds) Hanbook of plant cell culture ornamaental species. Mac. Grow. Hill. N.Y. Vol 5; 228 – 252.
George E.F. Hall M.A. and de Kerk G.J. 2008. The component of plant tissue culture media I, macro and micro nutrients . In George E.F. Hall M.A. and de Kerk G.J. (eds) Plant propagation by tissue culture the background vol I 3rd ed. Springer Netherlands p. 274 – 338.
Gopal J and Garg. I.D 2011. An efficient protocol of chemo thermotheraphy for elimination of potato (Solanum tuberosum L) virus by meristem tip culture. Ind. Agr. Sci, 544- 549.
Haque, M.E. and Mansfied, J.W. 2004. Effect of genotype and explant age on callus induction and subsequent plant regeneration from root derived callus of indica rice genotypes. Plant cell tissue org. cult. Vol 78 ,pp 217 – 223.
Haque, M. A, Nath U.K. Ahmad Q.N, Alam M.S. 2003. Effect of 2,4 and BAP on in vitro regeneration of garlic (Allium sativum L) on line J of Biological Sci. 2 (12), 771 -774.
Jena R.S, Samal K.C. 2011. Endogenous microbial contamination during in vitro culture of sweet potato (Ipomoea batatas L). Identivication and prevention. In J. Of Agric Tech. Vol 7 (6), 1725 -1731.
Kapoor R, Nasrin S.A. Mahmooduzzafar and Mujib. A. 2011. Establishment of efficient method for callus culture and shoot regeneration of local Indian garlic (var. Jamunsafed). J. of Ecobio technology 3 (12). 14 – 17.
Kamstaityte. D and Stanys. 2004 Micropropagaion of onion (Allium cepa L). Acta Univ. Latv. Biol. 676; 173 -176
Klein R and Livingstone C. 1983. Eradication of virus x and S from potato shoot tip culture with ribavirin. Phytophatology. Vol 73, 1049 – 1050.
Lee S.Y. ; Kim H.H; Kim Y.K; Park N.I. and Park S.U. 2009. Plantregeneration of garlic (Allium sativum L) via somatic embryogenesis. Sci. Res and Essay. Vol. 4 (a3); 1569 – 1574.
Luc and Bridgen M.P. 1996. Effect of genotype, culture medium and embryo developmental stage on the in vitro respons from ovuless cultures of interspesific hybrids of altstroemenia. Plant Sci. vol. 116 ; 205 – 212.
Metwally E.L, Denery M.E, Dewir T.H, Naido Y. 2014. In vitro propagation of garlic (Alium sativum L) through adventitious shoot organogenic . African J. of Biotech. 13 (38). 3892- 3900
Naik P.S. and Chandra R. 1993. Use of tissue culture tecnique in crop improvement with special reference to potato – CPRI Shinla , 10 pp
regulator 11; 337 – 383.
Oana D, Erdie L, Danci V, Anca M, David B and F Berbentea. 2009. Influence of ribavirin on potato plants regeneration and virus eradication. J. Hort. Forestry and Biotech. Vol 13; 421 – 425.
Okta S; Kumiga T; Nishikawa F; Yamasaki A; Endo T; Iwani T and Yoshida T. 2011 Evaluation of novel antiviral agent in the elimination of satsumo Dwarf (SDV) by micrografting in citrus. J. japan. Soc. Hort. Sci. 80; 145 – Noiton D; vine J.H and Mullins M.G. 1992. Effect of serial sub culture in vitro on the endogenous level of Indole -3 Acetic Acid and Abscisic Acid and root ability in micro cutting of “Jonathan Apple“. Plant growth 149
Pramesh, D, V.K. Baranwal 2015. Production of virus free garlic (Allium sativum L) through meristem tip culture after solar or hor treatment of cloves . J. Hort Sci & Biotech 90 (2). 180 – 186.
Roksana R, M.F. Alam, R. Islam and M.M Hosain 2002. In vitro bulblet formation from shoot apex in garlic (Allium Sativum L). Plant tissue culture 12 ; 11 -17
Sastry and Zitter T. 2014. Management of virus and viroid diseases of crops in the tropic. In SasTry K.S. Zitter T.A. Plant virus and viroid diseases in the tropic, epidemiology and management. Springer Vol 2 149 – 480.
Sidewell ,R.W , Huffman, JH Share, GP Allen, Lb Witkowski J.T and Robins R.K . 1972. Broad spectrum antiviral activity of virazole 1-β. D – Ribofuralnosyl -1, 2,4 –Triazole-3-carboxamide. Sci. 177 ; 705 -706.
Taskin H, Bahtemur G, Kurul M, Buyukalak S. 2013. Use of tissue culture technique for producing virus free plant in garlic and their identification through real time PCR. The Scientific world J. Hindawi. Vol 2013; 1-5.
Verma, N ram and Zaidi A 2005. In vitro production of prunus nectrotic ringspot virus free bgonianns through chemo and thermotheraphy. Sci. Hor. Vol 103; 239 – 247.
Walkey D.G.A; Webb M.J.W; Bold C.J. and Miller A. 1987.Production of virusfree garlic (Allium sativum L) and shallot (Allium ascolonicum L) by meristem tip culture. J. Hort Sci. 62 ; 211 – 219.
Wattimena, G.A; Nurhayati M; Armini N.M; Purwito A; Efendi D; Purwoko B.S and Khumaida N. 2011. Biotek nologi dalam Pemuliaan tanaman IPB. Press Bogor.
Ammissah S, Collenmann P, Sintim H, Akromad R. 2016. In vitro control of microbial contamination of sweet potato cultured with nodal explants. In Annual Res. &Riview in Biology . vol 9 (3) 1-8.
Aqlima, Bambang S.P, Sri H.H dan Dinny D. 2018. Aplikasi ribavirin pada shoot tip bawang merah untuk eliminasi virus OYDV . J.Hort vol 28; 251 -258.
Armin , N.M; G.A. Wattimena; L. Winata 1992. Perbanyakan tanmana dalam bioteknologi tanaman dalam Wattimena et al (eds) PAU Bioteknologi IPB. Dirjen Dikti Dept. P&K. pp 12 – 18
Asih K.K and Neni Gunaeni. 2018. efek antiviran ribavirin dalam pertumbuhan dan perkembangan eksplant bawang putih cv. Lumbu hijau, cv Lumbu kuning dan cv. Tawangmangu. Agrin Vo. 22, P. 93 -103. ISSN 1410-0029.
Awan, A.R; Mughal S.M; Iftilhar Y and Kan. H.Z. 2007 . In vitro elimination of potato leaf roll virus from potato varieties. Euro. J. R. 18; 154 – 164.
Ayabe M. and Sumi S. 1988. Establishment of a novel tissue culture method , stem disc culture and its practical application to micropropagation of garlic (Allium sativum L). Plant cell culture Report. 17; 773 -779.
Bhojwani S.S and P.K. Datu. 2013. Production of virus free plants by tissue culture pp. 227 – 243. In S.S Bhojwani and P.K. datu (eds). Plant tissue culture. An Introduction to text. Springer India. DOI. 10 1007/979-81-322-1026-9
Bodani A and Chamka J.S. 2010. In vitro sterilization protocol for micropropagation of solanum tuberosum cv. Kufri Himalini . Academia Arena 2 ( 4) pp 24 – 27.
Dinarti D; Agus Purwito; Anas D,S dan Iis. R; 2008. Pembentukan umbi lapis mikro dua kultivar bawang merah (Allium cepa var Agregatum group). Pada beberapa konsentrasi sucsinic Acid Daminozide Hydrazide. J. Ilmu Pertanian Indonesia. April 2008; 32 – 37. ISSN 0853 – 4217.
Dugssa G and Feyissa T. 2011. In vitro production of virus free sweet potato (Ipomoe batatas L) by meristem culture and thermotheraphy. Etiop. J. Sci. 34 (1) 17 – 28.
Gamborg O.L. et al. 1976. Nutrient requirement of suspension culture of soybean root. Cell Exp. Res. 50 151 – 58.
Geier, T. 1990. Anthorium . In Ammirato , P.V; D.A. Evans; W.R. Sharp and Y.P. S. Bajaj (eds) Hanbook of plant cell culture ornamaental species. Mac. Grow. Hill. N.Y. Vol 5; 228 – 252.
George E.F. Hall M.A. and de Kerk G.J. 2008. The component of plant tissue culture media I, macro and micro nutrients . In George E.F. Hall M.A. and de Kerk G.J. (eds) Plant propagation by tissue culture the background vol I 3rd ed. Springer Netherlands p. 274 – 338.
Gopal J and Garg. I.D 2011. An efficient protocol of chemo thermotheraphy for elimination of potato (Solanum tuberosum L) virus by meristem tip culture. Ind. Agr. Sci, 544- 549.
Haque, M.E. and Mansfied, J.W. 2004. Effect of genotype and explant age on callus induction and subsequent plant regeneration from root derived callus of indica rice genotypes. Plant cell tissue org. cult. Vol 78 ,pp 217 – 223.
Haque, M. A, Nath U.K. Ahmad Q.N, Alam M.S. 2003. Effect of 2,4 and BAP on in vitro regeneration of garlic (Allium sativum L) on line J of Biological Sci. 2 (12), 771 -774.
Jena R.S, Samal K.C. 2011. Endogenous microbial contamination during in vitro culture of sweet potato (Ipomoea batatas L). Identivication and prevention. In J. Of Agric Tech. Vol 7 (6), 1725 -1731.
Kapoor R, Nasrin S.A. Mahmooduzzafar and Mujib. A. 2011. Establishment of efficient method for callus culture and shoot regeneration of local Indian garlic (var. Jamunsafed). J. of Ecobio technology 3 (12). 14 – 17.
Kamstaityte. D and Stanys. 2004 Micropropagaion of onion (Allium cepa L). Acta Univ. Latv. Biol. 676; 173 -176
Klein R and Livingstone C. 1983. Eradication of virus x and S from potato shoot tip culture with ribavirin. Phytophatology. Vol 73, 1049 – 1050.
Lee S.Y. ; Kim H.H; Kim Y.K; Park N.I. and Park S.U. 2009. Plantregeneration of garlic (Allium sativum L) via somatic embryogenesis. Sci. Res and Essay. Vol. 4 (a3); 1569 – 1574.
Luc and Bridgen M.P. 1996. Effect of genotype, culture medium and embryo developmental stage on the in vitro respons from ovuless cultures of interspesific hybrids of altstroemenia. Plant Sci. vol. 116 ; 205 – 212.
Metwally E.L, Denery M.E, Dewir T.H, Naido Y. 2014. In vitro propagation of garlic (Alium sativum L) through adventitious shoot organogenic . African J. of Biotech. 13 (38). 3892- 3900
Naik P.S. and Chandra R. 1993. Use of tissue culture tecnique in crop improvement with special reference to potato – CPRI Shinla , 10 pp
regulator 11; 337 – 383.
Oana D, Erdie L, Danci V, Anca M, David B and F Berbentea. 2009. Influence of ribavirin on potato plants regeneration and virus eradication. J. Hort. Forestry and Biotech. Vol 13; 421 – 425.
Okta S; Kumiga T; Nishikawa F; Yamasaki A; Endo T; Iwani T and Yoshida T. 2011 Evaluation of novel antiviral agent in the elimination of satsumo Dwarf (SDV) by micrografting in citrus. J. japan. Soc. Hort. Sci. 80; 145 – Noiton D; vine J.H and Mullins M.G. 1992. Effect of serial sub culture in vitro on the endogenous level of Indole -3 Acetic Acid and Abscisic Acid and root ability in micro cutting of “Jonathan Apple“. Plant growth 149
Pramesh, D, V.K. Baranwal 2015. Production of virus free garlic (Allium sativum L) through meristem tip culture after solar or hor treatment of cloves . J. Hort Sci & Biotech 90 (2). 180 – 186.
Roksana R, M.F. Alam, R. Islam and M.M Hosain 2002. In vitro bulblet formation from shoot apex in garlic (Allium Sativum L). Plant tissue culture 12 ; 11 -17
Sastry and Zitter T. 2014. Management of virus and viroid diseases of crops in the tropic. In SasTry K.S. Zitter T.A. Plant virus and viroid diseases in the tropic, epidemiology and management. Springer Vol 2 149 – 480.
Sidewell ,R.W , Huffman, JH Share, GP Allen, Lb Witkowski J.T and Robins R.K . 1972. Broad spectrum antiviral activity of virazole 1-β. D – Ribofuralnosyl -1, 2,4 –Triazole-3-carboxamide. Sci. 177 ; 705 -706.
Taskin H, Bahtemur G, Kurul M, Buyukalak S. 2013. Use of tissue culture technique for producing virus free plant in garlic and their identification through real time PCR. The Scientific world J. Hindawi. Vol 2013; 1-5.
Verma, N ram and Zaidi A 2005. In vitro production of prunus nectrotic ringspot virus free bgonianns through chemo and thermotheraphy. Sci. Hor. Vol 103; 239 – 247.
Walkey D.G.A; Webb M.J.W; Bold C.J. and Miller A. 1987.Production of virusfree garlic (Allium sativum L) and shallot (Allium ascolonicum L) by meristem tip culture. J. Hort Sci. 62 ; 211 – 219.
Wattimena, G.A; Nurhayati M; Armini N.M; Purwito A; Efendi D; Purwoko B.S and Khumaida N. 2011. Biotek nologi dalam Pemuliaan tanaman IPB. Press Bogor.
